1. Zeiss LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System
Coupled with: Zeiss Inverted Axiovert 200 M Microscope Zeiss CTI Controller 3700 Zeiss Tempcontrol 37-2 Digital
(1) Laser Lines Argon: 458/477/488/514 nm, 25mW HeNe1: 543 nm, 1mW HeNe2: 633 nm, 5mW Chameleon XR Laser System: tunable, 705 nm – 980 nm
Chameleon laser excites fluorophores from Blue to Red range, but most efficient with UV and near-UV excitable dyes.
(2) Objectives
Objectives available for Zeiss Axiovert 200 M inverted microscope: Mag.
| Objective
| NA
| Medium
| WD(mm)
| For 2-Photon
| 10x
| Plan-Apochromat
| 0.45
| Air
| 2.10
| No
| 20x
| Plan-Apochromat
| 0.75
| Air
| 0.61
| Yes
| 40x
| Plan-Neofluar
| 1.30
| Oil (DIC)
| 0.17
| Yes
| 63x
| C-Apochromat
| 1.20
| Water (Corr.)
| 0.24
| No
| 100x
| Alpha Plan-Fluar
| 1.45
| Oil
| 0.10
| No
|
(3) Fluorescence Filter Sets
Fluorescence filter sets available for use with the Zeiss Axiovert 200 M inverted microscope:
2. Olympus BX61WI InVivo Two-Photon Confocal Microscope
(1) Laser Line - Coherent Chameleon XR Laser System: tunable, 705 nm – 980 nm
(2) Objectives Objectives available for Olympus BX61WI upright microscope: Mag. | Objective | NA | Medium | WD(mm) | For 2-Photon | 10x | UMPLFL | 0.30 | Water | 3.30 | No | 60x | LUMPLFL (IR2) | 0.90 | Water | 2.00 | Yes |
(3) Filter Sets Fluorescence filter sets available for Two-Photon imaging: - GFP/DsRed Filter Set (Dichroic mirror: T560LPXR):
Channel 1: GFP (ET510 - 80m 2p) –Emission filter: 470-550 nm Channel 2: dsRed (ET620 - 60m 2p) –Emission filter: 590-650 nm
- CFP/YFP Filter Set (Dichroic mirror: T505LP):
Channel 1: CFP (ET480 - 40m 2p) –Emission filter: 460-500 nm Channel 2: YFP (ET535 - 30m 2p) –Emission filter: 520-550 nm
3. Image Processing Software 4. Preparative Equipments
(1) Cell Culture Room |
