Instrumentation

HPLC

Analytical/Preparative HPLC is performed on a Waters Delta Prep HPLC System with Empower software. This system is set up for the reverse phase chromatography of peptides.

Mass Spectrometry

Thermo Fisher Scientific LTQ Orbitrap XL ETD. This is the most powerful instrument for proteomics analyses that is available to our customers. The instrument combines high resolution (up to 100,000) and high mass accuracy (<5ppm) with the high speed of an LTQ. The instrument is equipped with three different and complementary fragmentation techniques CID, HCD, and ETD (this method will be installed later in the year). It is the most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantitation via stable isotope labelling such as iTRAQ or label-free quantitation.

Applied Biosystem Voyager DE-STR workstation. The instrument is a floor standing MALDI-TOF (matrix assisted laser desorption Ionization /time-of-flight) mass spectrometer . It features delayed extraction in linear and reflector modes of operation for low and high resolution acquisitions. It has a mass range in excess of 300 kDa, and sensitivity to less than 5 fM. It is mainly used for the mass analysis of peptides, proteins, DNA and the identification of proteins by Peptide Mass fingerprinting (PMF).

Thermo Fisher Scientific LTQ XL. This mass spectrometer is mainly used for our shot-gun proteomics approaches, where the fast MS/MS scan speed allows to identify more peptides in a given time. It also comes with multiple dissociation techniques, PQD, ETD and CID. Pulsed Q Collision Induced Dissociation (PQD) is a patented technique that eliminates the low mass cut-off concern inherent with all ion traps.

Thermo Fisher Scientific TSQ Quantum Access. This mass spectrometer is a triple quadrupole mass spectrometer used for quantitative as well as qualitative work. It is equipped with two different ion sources, ESI and APCI, which allow us to analyze different types of samples. The instrument is mainly used to analyze DNA, peptides, proteins and small molecules.

BIACORE 2000

The BIACORE 2000 is an instrument which can be used by individual investigators. The instrument is designed to perform basic kinetics, heterogeneous ligand analysis, heterogeneous analyte analysis, epitope mapping, multi-site binding, concentration analysis, and other applications.

Gel Electrophoresis/Proteomics

2-D Gel Electrophoresis (Bio-Rad)

Imaging and Analysis of 2-D Gels using the Versa Doc Imaging System (Bio-Rad) and PDQuest Advanced ver.8.0 2-D Analysis Software (Bio-Rad)

Automated excision of protein spots from gels using the Proteome Works Plus Spotcutter (Bio-Rad)

Automated tryptic digestion using the Multi Probe II HT EX (Perkin Elmer)